Jackie Henderson has left the Kyle and Jackie O Show after 22 years following an on-air argument between her and Kyle Sandilands during the program on February 20.

ARN Media also announced this afternoon that Sandilands could be out of the job if he does not prove within 14 days that he did not breach his contract for an alleged “act of serious misconduct” on that fateful show.

Henderson has not returned to work since the show and has remained silent on the issue.

Kyle and Jackie O were on air together for 22 years. (KIIS)

ARN Media’s statement reads: “Ms Jacqueline Henderson has given notice that she ‘cannot continue to work with Mr Kyle Sandilands’.

“Accordingly, ARN has terminated the services agreement with Henderson Media Pty Ltd, under which Ms Henderson presents the Kyle and Jackie O show. Ms Henderson will cease to present the Kyle and Jackie O show.

“ARN has also offered to Ms Henderson the possibility of an alternative show on the ARN network. ARN has also provided written notice to Mr Kyle Sandilands and Quasar Media Services Pty Ltd (Quasar Media) stating that it considers that Mr Sandilands’ behaviour during the show on 20 February 2026 is an act of serious misconduct which is in breach of ARN’s services agreement with Quasar Media, under which Mr Sandilands presents the Kyle and Jackie O show.

“Mr Sandilands has been given 14 days to remedy this breach. If it is not remedied, ARN will terminate the services agreement with Quasar Media, and in that event Mr Sandilands will cease to present the Kyle and Jackie O show.

“During the 14 day period, Mr Sandilands will not take part in the show. The KIIS breakfast show will be taken off-air effective immediately, with interim arrangements made for the show.”

This week marked the second week off-air for Henderson, following the February 20 broadcast that ended in tears after Sandilands called out her work ethic and teased her “obsession” with astrology.

Today FM radio presenters Kyle and Jackie O at their studio in Bondi Junction in 2005. (Lee Besford)

The on-air fight began after Jackie O read from Andrew Mountbatten-Windsor’s astrology birth chart and Sandilands complained about her interest in astrology charts.

“Your fixation on this has made you almost unworkable,” Sandilands told her.

“You are off with the fairies with this s–t, it’s mental.”

He claimed her fixation on astrology charts made her sound like a “drug addict”.

Henderson has previously opened up on air about addiction issues that led to her going to rehabilitation in the US in 2022.

Henderson said Sandiland’s accusations were “so unfair” and that he was attacking her on air.

“I would never say things like that about you,” she said.

“It’s a total attack, it really is. Kyle, to say ‘you don’t know what the F is going on, you’re not doing your job,’ that’s an attack and I wouldn’t do that.”

Sandilands and Henderson have been in an on air partnership for 27 years.

They moved to breakfast in 2005 and then to KIIS in 2014.

Then have been under contract in a lucrative $200m 10-year deal that was set to run until December 2034.